The metabolism of C2 compounds in micro-organisms. 7. Preparation and properties of crystalline tartronic semialdehyde reductase.
نویسندگان
چکیده
The biosynthesis of cell materials by Pseudomonas spp. growing on glycollate (Kornberg & Gotto, 1959, 1961), on glycine (Callely & Dagley, 1959), on oxalate (Quayle & Keech, 1959) or on other substrates catabolized to glyoxylate (Gray, Gerhart & Brooke, 1959) has been shown to be effected by a sequence of reactions in which, initially, glyoxylate is converted into glycerate. This transformation is achieved by the successive action of two adaptively formed enzymes: glyoxylate carboligase (Krakow & Barkulis, 1956; Krakow, Hayashi & Barkulis, 1959; Krakow, Barkulis & Hayashi, 1961), which catalyses the condensative decarboxylation of glyoxylate to tartronic semialdehyde:
منابع مشابه
Tartaric Acid Metabolism T7111. CRYSTALLISE TARTRONIC SEMIALDEHYDE REDUCTASE
Tartronic semialdehyde reductase from Pseudomonas putida has been purified and crystallized. As calculated from hydrodynamic measurements, the active enzyme has a molecular weight of 104,000 and appears to be composed of four subunits, each of which has a molecular weight of approximately 26,000. The enzyme catalyzes the reversible reduction of tartronic semialdehyde to D-glycerate in the prese...
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عنوان ژورنال:
- The Biochemical journal
دوره 81 شماره
صفحات -
تاریخ انتشار 1961